Lin XIANFENG, et al : Bone Glue -- articles & 20 patents [**rexresearch**](http://rexresearch.com/)[**rexresearch1**](http://rexresearch1.com) --- **Lin XIANFENG, et al** **Bone Glue** --- [**https://interestingengineering.com/science/chinas-oyster-inspired-bone-glue**](https://interestingengineering.com/science/chinas-oyster-inspired-bone-glue)**Chinaas oyster-inspired abone gluea bonds fractures, can replace metal in surgery** New bio-glue mimics oysters, fixing shattered bones in 3 minutes without major surgery. ...Chinese researchers has successfully created a new kind of medical adhesive called aBone-02.a Inspired by oysters, the new glue is designed to repair broken bones quickly without the need for metal plates, screws, or big surgeries. The new glue can be injected directly into a fracture site to help speed up bone repair. It bonds bone fragments together in 2a3 minutes, even in blood-rich areas where most adhesives fail. --- [**https://www.globaltimes.cn/page/202509/1343380.shtml**](https://www.globaltimes.cn/page/202509/1343380.shtml)**China introduces new abone gluea inspired by oysters for fast fracture repairing** A Chinese research team in East China's Zhejiang Province unveiled an innovative product called "Bone 02" bone glue on Wednesday. Inspired by oysters, this glue can treat fractures with a single injection and bond shattered bone fragments in just three minutes, according to local media Zhejiang Online. The team leader, Lin Xianfeng, an associate chief orthopedic surgeon at Sir Run Run Shaw Hospital affiliated with the renowned Zhejiang University, said the adhesive can achieve precise fixation within two to three minutes, even in a blood-rich environment. In one trial case, the procedure was completed in less than three minutes a whereas traditional treatment would have required a large incision to implant steel plates and screws, Zhejiang Online said. Laboratory tests confirmed that 'Bone-02' performed strongly in both safety and effectiveness. It demonstrated a maximum bonding force of over 400 pounds, a shear strength of about 0.5 MPa, and a compressive strength of around 10 MPa. These properties suggest it has the potential to replace traditional metal implants, while also reducing the risks of foreign-body reactions and infection, said the report. Another feature of 'Bone-02' is that it can be naturally absorbed by the body as the bone heals, eliminating the need for a second surgery to remove implants, local media reported. In 2016, while still a resident physician, Lin observed that even the most experienced surgeons needed hours in the operating room to fix shattered bone fragments, and the results were often far from ideal, according to Zhejiang Online. Drawing on his clinical experience and research, Lin decided to lead a team to develop a "bone glue." He later found inspiration after observing oysters clinging firmly to a bridge underwater. The observation sparked the idea of developing a "bone glue" with similar properties in the human body's moist environment - an idea his team eventually turned into reality, the report said. --- [**https://www.sixthtone.com/news/1017606**](https://www.sixthtone.com/news/1017606)**For a Team of Chinese Scientists, the Oyster Is Their World***Inspired by oystersa natural grip, a team of researchers from Zhejiang have created a bio-glue to fuse shattered bones, a world first.*By Jiang Xinyi ...In 2010, a U.S. research team at Purdue University first characterized the unique agluea containing protein and calcium carbonate. --- [**https://pubs.acs.org/doi/abs/10.1021/ja104996y**](https://pubs.acs.org/doi/abs/10.1021/ja104996y)**Oysters Produce an OrganicaInorganic Adhesive for Intertidal Reef Construction****Jeremy R. Burketta Lauren M. Highta Paul Kennya!Jonathan J. Wilker** ... This cement is an organicainorganic hybrid and differs from the surrounding shells by displaying an alternate CaCO3 crystal form, a cross-linked organic matrix, and an elevated protein content. Emerging themes and unique aspects are both revealed when comparing oyster cement to the adhesives of other marine organisms. The presence of cross-linked proteins provides an analogy to mussel and barnacle adhesives whereas the high inorganic content is exclusive to oysters. With a description of oyster cement in hand we gain strategies for developing synthetic composite materials as well as a better understanding of the components needed for healthy coastal environments. --- [**https://worldwide.espacenet.com**](https://worldwide.espacenet.com) [**WO2025146088 -- NANOMATERIAL FOR PREVENTING TUMOR BONE METASTASIS, PREPARATION METHOD THEREFOR, AND USE THEREOF**](WO2025146088tr.pdf) The present invention relates to a nanomaterial for preventing tumor bone metastasis, a preparation method therefor, and use thereof. The present invention has discovered a spatiotemporal coupling interaction between tumor cells and osteoclasts, and provides a tumor-bone initial metastasis behavior-targeting strategy which can accurately prevent tumor metastasis on the basis of the source, and can avoid drug resistance and biochemical drug resistance. On this basis, the present invention designs a physical killing nanomaterial targeting tumor-osteoclast conjugates, the nanomaterial being a bone targeting group-modified nanovesicle encapsulating a carbonate compound and a phosphate compound. The nanomaterial can be effectively concentrated in bone tissue; when tumor cells are activated, the acid secretion function of tumor-related osteoclasts in nearby tumor-osteoclast conjugates which are in contact with the tumor cells triggers the carbonate compound to generate carbon dioxide gas and promotes the release of the phosphate compound, which forms calcium phosphate crystals with calcium ions so as to kill nearby tumor cells, thus achieving a specific very-early tumor metastasis inhibition effect. [**CN118662693 -- Bionic bone substance with controllable coagulation time as well as preparation method and application of bionic bone substance**](CN118662693tr.pdf) The invention belongs to the field of bone repair biological materials, and discloses a bionic bone substance with controllable coagulation time, the bionic bone substance comprises calcium phosphate, a phosphate-based organic matter and a bionic spongy reaction agent, the bionic spongy reaction agent comprises a spongy reaction liquid phase and a spongy reaction solid phase, the spongy reaction liquid phase comprises water, sodium citrate and citric acid, and the spongy reaction solid phase comprises calcium phosphate, phosphate-based organic matter and bionic spongy reaction solid phase. The solid phase of the loosening reaction is sodium bicarbonate. The bionic bone can effectively control the curing time and the cancellous degree, different proportions are selected according to the clinical operation time requirement and the cancellous condition of the bone of a clinical filling part, and the bionic bone is easy to use clinically. **[CN116328039 -- Specific-mineralization-degree natural bone repair material capable of regulating inflammation metabolism...](CN116328039%20tr.pdf)** The invention discloses a specific-mineralization-degree natural bone repair material capable of regulating and controlling inflammation metabolism and a preparation method and application of the specific-mineralization-degree natural bone repair material. The material is a mineralization extracellular matrix formed after bone tissue ultrasonic decellularization and demineralization; the mineralization degree is that the mass content of calcium is 10-20%. The method specifically comprises the following steps: cutting natural bones into bone slices, cleaning, processing the bone slices into different specifications, cleaning, carrying out disinfection treatment, degreasing treatment, decellularization treatment and specific demineralization treatment until the mass content of calcium is 10-20%, carrying out last cleaning, detecting residues, freeze-drying, packaging and sterilizing. The material has surface charged groups, can realize selective permeation of metabolites so as to regulate and control inflammation, has surface collagen residues, can promote cell adhesion, and has a remarkable bone repair promoting capability. [**CN116019779 -- Osteoporosis treatment delivery system based on osteoclast precursor cell targeting circBBS9 knock-down**](cn116019779tr.pdf) The invention discloses an osteoporosis treatment delivery system based on osteoclast precursor cell targeting circBBS9 knock-down. According to the osteoclast precursor cell targeting circBBS9 knock-down, circRNA is inhibited by siRNA in osteoclast precursor cells, so that osteoclast polynucleation and bone resorption capability weakening are achieved, and related functions of the osteoclast precursor cells are reserved. Meanwhile, osteoclast precursor cell targeting delivery is conducted on the siRNA through cell membrane microvesicles homologous to osteoclast precursor cells, the effect of treating osteoporosis is achieved, the preparation method and application of the osteoclast precursor cell targeting siRNA are provided, and the technical problem that an existing treatment mode cannot selectively inhibit the specific cell stage in an osteoclast lineage is solved. [**CN116024210 -- Osteoporosis marker circRNA and application thereof**](CN116024210tr.pdf) The invention discloses an osteoporosis marker circRNA and application thereof, and belongs to the technical field of biology. The invention proves that the expression quantity of circBBS9 and human homologues thereof in osteoporosis is increased. Meanwhile, a siRNA (small interfering Ribonucleic Acid) capable of knocking down circBBS9 expression is designed. The invention proves that the miR-423-3p is a downstream target of circBBS9 (circBBS9). The expression of the circBBS9 in osteoclast polynucleation is rapidly increased, differentiation of osteoclast mononuclear precursors is not affected by inhibition of the circBBS9, the osteoclast polynucleation level and bone resorption capacity can be weakened, and the bone density of osteoporosis mice is increased. The siRNA prepared by the invention can be used as a medicine for preventing and treating osteoporosis. **[WO2023040853 -- PERIOSTEUM-BONE COMPLEX FOR RECONSTRUCTING SOFT TISSUE-BONE IMMUNE REPAIR](wo2023040853.pdf)** A periosteum-bone complex for reconstructing a soft tissue-bone immune repair environment and a preparation method therefor. The preparation method comprises: cutting and proofing, repeatedly rinsing with deionized water, freezing and thawing with liquid nitrogen, performing ultrasonic decalcification, and treating with a PBS buffer solution containing a protease inhibitor, a PBS buffer solution containing Triton X-100, a PBS buffer solution containing SLES, a PBS buffer solution containing DNase I and a Tris-HCl buffer solution to obtain a cell-free periosteum-bone complex material. The complex retains a good periosteum-bone space structure and extracellular active ingredients, provides a "perichondrium-sclerotin" biphasic interface, effectively blocks excessive inflammation infiltration of a soft tissue source, participates in early immune remodeling of soft tissue-bone injury, and forms a benign immune-repair microenvironment by combining its own osteogenesis and vascularization advantages of the complex. A new way of thought is provided for promoting bone tissue regeneration and repair by using a biological material. [**US2022313609 -- Nano composite material aiming at acidic sealing zone in osteoclasts and preparation method thereof**](US2022313609A1.pdf) A nano composite material aiming at an acidic sealing zone in osteoclasts and a preparation method thereof are provided. The nano composite material aiming at the acidic sealing zone in the osteoclasts includes a nano material, bone-targeting molecules, and a compound able to react with the acidic sealing zone in the osteoclasts, wherein: after being modified by the bone-targeting molecules, the nano material is loaded with the compound able to react with the acidic sealing zone in the osteoclasts. Through accurate mature osteoclast targeting and chemically regulated biocascade effects, the osteoclasts are inhibited, which provides a new idea and a new tool for drug therapy of abnormal osteoclast activation. [**CN113577391 -- Preparation method of natural tissue-derived epiphyseal cartilage combined bone acellular material**](CN113577391tr.pdf) The invention discloses a preparation method of a natural tissue-derived epiphyseal cartilage combined bone acellular material, and belongs to the technical field of biological materials for tissue or organ repair and regeneration. The preparation method comprises the following steps: rinsing and repeatedly freezing and thawing epiphyseal cartilage combined bone at the distal femur of a young large white pig, and treating the epiphyseal cartilage combined bone with a PBS buffer solution containing TritonX-100, a PBS buffer solution containing SLES, a PBS buffer solution containing HTHOPS, a PBS buffer solution containing DNaseI and normal saline to obtain the decellularized epiphyseal cartilage combined bone material. After thorough decellularization treatment is carried out on the epiphyseal and cartilage combined bone material, the completeness of the epiphyseal and cartilage combined bone ECM is reserved to the maximum extent while cells with immunogenicity and cell content components are completely removed, and the epiphyseal and cartilage combined bone ECM has the capability of repairing osteochondral defects and can be used for treating orthopedic diseases caused by osteochondral defects. [**US11684696 -- Preparation method of gradient mineralized cancellous bone matrix material**](US2021121605A1.pdf) A gradient mineralized cancellous bone matrix material and a preparation method thereof are provided, and the preparation method includes: processing naturally-derived bone tissue with an immunogenicity removal treatment for decellularization, and processing an obtained decellularzed bone with a gradient demineralization treatment to obtain the gradient mineralized cancellous bone matrix material. The present invention expands a porosity of the bone matrix material and a collagen exposure degree on a surface thereof, which effectively releases growth factors and improves adhesion of the material to the cells, so as to up-regulate genes and proteins related to cell regeneration. The present invention not only retains the biomechanical properties and three-dimensional microstructure of natural bone ECM scaffolds, but also plays an active role for osteogenesis, angiogenesis and collagen mineralization in the early stage of fracture, thereby increasing engraftment adhesion of cells and promoting differentiation induction of cells. [**CN112618797 -- Preparation method of antibiotic-crosslinked specific demineralized extracellular matrix scaffold**](CN112618797tr.pdf) The invention discloses a preparation method of an antibiotic-crosslinked specific demineralized extracellular matrix scaffold, and belongs to the technical field of bone infection treatment. The invention discloses the preparation method of the antibiotic-crosslinked specific demineralized extracellular matrix scaffold, which comprises the following steps of by using a specific demineralized and decellularized cancellous bone extracellular matrix scaffold (SDECM) as a substrate, carrying out compound drug loading on the substrate and the antibiotics in two forms of electrostatic adsorption and chemical crosslinking, releasing antibiotics in two modes of rapid pH response and accompanying material degradation in an acid environment, and adopting the antibiotics for resisting infection and promoting repair through SDECM, so that the pH-sensitive release drug in the early acidic environment of infection is rapidly sterilized, the drug is slowly released in the middle-late physiological environment for continuous sterilization, the treatment effect of permanent sterilization capability and prevention of infection recurrence is still achieved in the absence of infection, and a new thought and a new tool are provided for antibacterial and repair treatment of bone infection. [**CN111481678 -- Nano material for osteoclast acidic closed region and preparation method thereof**](CN111481678tr.pdf) The invention discloses a nano material for an osteoclast acidic closed region and a preparation method thereof. The nano material for the osteoclast acidic closed region comprises a nano material, bone targeting molecules and a compound which generates a chemical reaction on the osteoclast acidic closed region, after the nano material is subjected to bone targeting molecular modification, a compound which generates a chemical reaction on an osteoclast acidic region is entrapped; according to the nano material, osteoclasts are inhibited through accurate mature osteoclast targeting and physiological chemical reaction regulated biological cascade reaction, and a new thought and a new tool are provided for pharmaceutical treatment of osteoclast abnormal activation. [**CN110237303 -- Preparation method for decellularized periosteal matrix gel material sourced from natural tissue**](CN110237303tr.pdf) The invention discloses a preparation method for a decellularized periosteal matrix gel material sourced from natural tissue. By conducting freeze drying on decellularized periosteal matrix sourced from mammal, bioactive molecules are kept to the maximum degree; the decellularized periosteal matrix gel material sourced from natural tissue is ground into powder, the grain size of the powder is controlled, the contact area of a solution is increased, in this way, digestion is promoted, and finally the standard and uniform decellularized periosteal matrix gel having a good osteogenic activity potential is obtained. In addition, EDC crosslinking growth factors have a good promoting effect when regenerated bone tissue is regenerated excellently and grows into with vascularization. The decellularized periosteal matrix gel material can be used for repairing bone defects, bone disunion and other kinds of bone regeneration obstacles caused by all kinds of clinical pathogeneses. [**CN105879120 -- Preparation method of tendon conjunction bone decellularization material of natural tissue source**](CN105879120tr.pdf) The invention discloses a preparation method of a tendon conjunction bone decellularization material of a natural tissue source. The heel tendon conjunction bone tissue of any size of a mammal is treated by normal saline containing protease inhibitor, PBS containing protease inhibitor, a PBS buffer solution containing Triton X, a PBS buffer solution containing SDS and a PBS buffer solution containing DNA enzyme, and the tendon conjunction bone decellularization material is obtained. Complete decellularization treatment can be carried out on tendon cells and bone cells on tendon conjunction bone tissue and cartilage cells on the interface at the same time, conditions are mold, no ECM damage is caused, the process is rapid and stable, and the obtained tendon conjunction bone material has the advantages of being good in biocompatibility, strong in plasticity, high in biomechanical strength and the like, and can be used for repairing tendon degenerative diseases caused by various etiological factors clinically, tendon severe tear and tendon conjunction bone regeneration and repair obstacles. [**CN105664255 -- Method for preparing synchondrosis bone acellular materials from natural tissue origins**](CN105664255tr.pdf) The invention discloses a method for preparing synchondrosis bone acellular materials from natural tissue origins.The method particularly includes treating optional synchondrosis bone tissues of mammals by the aid of normal saline buffer solution with protease inhibitors, organic solvent solution, PBS (phosphate buffer solution) with Triton X, PBS with SDS (sodium dodecyl sulfate) and PBS with DNA (deoxyribonucleic acid) enzymes to obtain the synchondrosis bone acellular materials.The method has the advantages that the optional synchondrosis bone tissues of the whole bodies of the mammals are subjected to thorough decellularization treatment, so that the synchondrosis bone acellular materials with high homogeneity can be efficiently, conveniently and quickly obtained; the integrity of the original ECM (extra cellular matrixes) can be kept while allogenic or xenogeneic cells with immunogenicity are removed, and the synchondrosis bone acellular materials can be applied to orthopedic diseases at optional positions of the whole bodies of the mammals. [**CN105435307 -- Natural-tissue-derived decellularized and decalcified bone material and preparation method thereof**](CN105435307tr.pdf) The invention discloses a preparation method of a natural-tissue-derived decellularized and decalcified bone material. According to the method, any bone tissue of a mammal is treated with a protease inhibitor-containing normal saline buffer, an organic solvent, Tirton X-containing PBS (phosphate buffer saline), SDS (sodium dodecyl sulfonate)-containing PBS, pancreatin-containing PBS, deoxyribonuclease-containing PBS, an EDTA (ethylene diamine tetraacetic acid) isotonic solution and ultrasonic waves, and the decellularized and decalcified bone material is obtained. Cancellous bones and cortical bones can be decellularized completely and simultaneously, the conditions are mild, damage to an ECM (extracellular matrix) is avoided, rapidness and stability are realized, and the obtained decellularized and decalcified bone material has the advantages of good biocompatibility, high plasticity, high biomechanical strength and the like and can be used for clinically repairing bone regeneration and repair disorder such as bone defects, nonunion and the like caused by various causes [**CN104511052 -- Culture method for composition of periosteal biological scaffold and allogenic seed cells**](CN104511052tr.pdf) The invention discloses a culture method for the composition of a periosteal biological scaffold and allogenic seed cells. The culture method comprises the following steps: the non-immunogenic periosteal biological scaffold and the seed periosteal cells are prepared; the non-immunogenic periosteal biological scaffold is sealed after low-temperature drying; after gamma-ray disinfection of the non-immunogenic periosteal biological scaffold, the seed periosteal cells, with the concentration of 1\*106 per milliliter, are dripped on the surface of the disinfected scaffold; a culture medium is added after the periosteal cells gradually permeate into the non-immunogenic periosteal biological scaffold together with a suspension, the medium change is carried out every 2 to 3 days, and the in-vitro composite culture lasts for one week. The periosteal biological scaffold obtained through the method has the characteristics of complete removal of immunogenic cells, as well as good preservation of the structure and major components of extracellular matrix; effective composition of the allogenic periosteal cells and the biological scaffold can be achieved, so that a biological composite scaffold material can be provided for tissue engineering researches on bone defects and bone nonunion. [**CN104307045 -- Decellularized periosteum material sourced from natural tissues and preparation method thereof**](CN104307045tr.pdf) The invention discloses a decellularized periosteum material sourced from natural tissues and a preparation method thereof. The method comprises the following steps: removing bony fragment of an extremity bone periosteum of an arbitrary mammal; rinsing for three times by aseptic PBS; oscillating for 1 hour by 5% PBS buffer solution containing 10KIU/ml protease inhibitor on a shaking table at 200rpm; adding mixed antibacterial solution into 5% PBS buffer solution containing TritonX-100, and oscillating for 48 hours on the shaking table at 250rpm; adding the mixed antibacterial solution into 10% PBS buffer solution containing SDS, and oscillating for 48 hours on the shaking table at 250rpm; and adding the mixed antibacterial solution into 1.5mg/ml PBS buffer solution containing DNAase, and oscillating for 12 hours on the shaking table at 250rpm. The decellularized periosteum material is wide in materials and can be produced in batches, and the prepared periosteum material has high safety. [**CN104307044 -- Natural tissue-derived total disc acellular material and preparation method thereof**](CN104307044tr.pdf) The invention discloses a natural tissue-derived total disc acellular material and a preparation method thereof. The natural tissue-derived total disc acellular material is prepared by virtue of the following steps: taking the intervertebral disc of a vertebrate, removing bone fragments, rinsing for 3 times by use of an aseptic PBS, and then shaking by 150rpm on a table concentrator for 4 hours in a PBS having the concentration of 10% and containing 10KIU/ml; adding a mixed antibacterial liquid to a PBS having the concentration of 4% and containing TritonX-100 and shaking by 150rpm on the table concentrator for 48 hours; adding the mixed antibacterial liquid to a PBS having the concentration of 5% and containing SDS and shaking by 150rpm on the table concentrator for 48 hours; finally, adding the mixed antibacterial liquid to a PBS having the concentration of 0.5mg/ml and containing a DNA enzyme and shaking by 150rpm on the table concentrator for 12 hours, and flushing by using the PBS for 1 hour. The preparation method of the natural tissue-derived total disc acellular material is wide in material source, high in safety and capable of realizing normal intervertebral disc reconstruction. [**WO2025146088 -- NANOMATERIAL FOR PREVENTING TUMOR BONE METASTASIS...**](WO2025146088tr.pdf) The present invention relates to a nanomaterial for preventing tumor bone metastasis, a preparation method therefor, and use thereof. The present invention has discovered a spatiotemporal coupling interaction between tumor cells and osteoclasts, and provides a tumor-bone initial metastasis behavior-targeting strategy which can accurately prevent tumor metastasis on the basis of the source, and can avoid drug resistance and biochemical drug resistance. On this basis, the present invention designs a physical killing nanomaterial targeting tumor-osteoclast conjugates, the nanomaterial being a bone targeting group-modified nanovesicle encapsulating a carbonate compound and a phosphate compound. The nanomaterial can be effectively concentrated in bone tissue; when tumor cells are activated, the acid secretion function of tumor-related osteoclasts in nearby tumor-osteoclast conjugates which are in contact with the tumor cells triggers the carbonate compound to generate carbon dioxide gas and promotes the release of the phosphate compound, which forms calcium phosphate crystals with calcium ions so as to kill nearby tumor cells, thus achieving a specific very-early tumor metastasis inhibition effect. [**US11684696 -- Preparation method of gradient mineralized cancellous bone matrix material**](US2021121605A1.pdf) A gradient mineralized cancellous bone matrix material and a preparation method thereof are provided, and the preparation method includes: processing naturally-derived bone tissue with an immunogenicity removal treatment for decellularization, and processing an obtained decellularzed bone with a gradient demineralization treatment to obtain the gradient mineralized cancellous bone matrix material. The present invention expands a porosity of the bone matrix material and a collagen exposure degree on a surface thereof, which effectively releases growth factors and improves adhesion of the material to the cells, so as to up-regulate genes and proteins related to cell regeneration. The present invention not only retains the biomechanical properties and three-dimensional microstructure of natural bone ECM scaffolds, but also plays an active role for osteogenesis, angiogenesis and collagen mineralization in the early stage of fracture, thereby increasing engraftment adhesion of cells and promoting differentiation induction of cells. [**US2022313609 -- Nano composite material aiming at acidic sealing zone in osteoclasts and preparation method thereof**](US2022313609A1.pdf) A nano composite material aiming at an acidic sealing zone in osteoclasts and a preparation method thereof are provided. The nano composite material aiming at the acidic sealing zone in the osteoclasts includes a nano material, bone-targeting molecules, and a compound able to react with the acidic sealing zone in the osteoclasts, wherein: after being modified by the bone-targeting molecules, the nano material is loaded with the compound able to react with the acidic sealing zone in the osteoclasts. Through accurate mature osteoclast targeting and chemically regulated biocascade effects, the osteoclasts are inhibited, which provides a new idea and a new tool for drug therapy of abnormal osteoclast activation. --- ---