Xin An ZENG [ ZENGA /ZHENG , &c. ] --- Electric Field
Sterilization & Chemical Acceleration

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**Xin An ZENG****[ ZENGA /ZHENG, &c. ], *et al*.**

**Electric Field Sterilization &
Chemical Acceleration**



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***New Scientist* # 2687**   
**17 December 2008**

**How to Make Cheap Wine Taste Like a Fine
Vintage**

How do you turn a bottle of cheap plonk into a fine vintage?

MOST people have got one lying around somewhere: a bottle of
cheap, nasty wine left over from a dinner party just waiting to
be offloaded on someone else - or quaffed late one night when
the good stuff has run out. But what if you could turn that
bargain-basement plonk into fine wine in minutes? In these
straitened times it could be just the thing a wine lover needs.

Traditionalists, of course, would insist that nothing can
replace genuine quality plus long, slow ageing in an oak barrel
and years of storage in cool, cobwebby cellars. But could there
be a short cut? Over the years, inventors have come up with
dozens of widgets that they claim can transform the undrinkable
or bring the finest wines to perfection without the long wait.
Sadly, there's little scientific evidence that most of them
work

Looks like you're stuck with the plonk.

Or are you? Fortunately, there is one technique that stands out
from the rest. It is backed by a decade of research, the results
have been published in a peer-reviewed journal and the end
product has passed the ultimate test- blind tasting by a panel
of wine experts. No fewer than five wineries have now invested
in the technology.

The secret this time is an electric field. Pass an undrinkable,
raw red wine between a set of high-voltage electrodes and it
becomes pleasantly quaffable. "Using an electric field to
accelerate ageing is a feasible way to shorten maturation times
and improve the quality of young wine," says Herve Alexandre,
professor of oenology at the University of Burgundy, close to
some of France's finest vineyards.

No matter how impatient or undiscriminating you may be, fresh
wine is undrinkable and can have horrible after-effects. Expect
an upset stomach, a raging thirst and the world's nastiest
hangover. The youngest a wine can be drunk is six months. Most,
especially reds, take longer to achieve the required balance and
complexity. The finest can take 20 years to reach their peak.

During ageing, wine becomes less acid as the ethanol reacts
with organic acids to produce a plethora of the fragrant
compounds known as esters. Unpleasant components precipitate out
and the wine becomes clearer and more stable. Red wines mellow
as bitter, mouth-puckering tannin moleculescombine with each
other and with pigment molecules to form larger polymers, at the
same time releasing their grip on volatile molecules that
contribute to the wine's aroma.

These reactions take time and need a small but steady supply of
oxygen. In barrel-aged wines, oxygen leaks through the wood,
while wine matured in steel tanks is often helped along by
introducing microscopic oxygen bubbles.

There are good commercial reasons why winemakers would love get
their hands on a speedier alternative, especially in places like
China where the industry is young and booming. It would allow
them to get their wines into the shops faster to meet
ever-increasing demand, and cut the cost of storage.

The food industry has experimented with electric fields as an
alternative to heat-treating since the 1980s, and 10 years ago   
  
**Xin An Zeng** , a chemist at the South China University of
Technology in Guangzhou, decided to see what he could do for
wine. Early results were promising enough for Zeng and his
colleagues to develop a prototype plant in which they could
treat wine with fields of different strengths for different
periods of time.

They pumped the wine through a pipe that ran between two
titanium electrodes, fed with a mains-frequency alternating
supply boosted to a higher voltage. For the test wine, the team
selected a 3-month-old cabernet sauvignon from the   
    
Suntime Winery, China's largest producer. Batches of wine spent
1, 3 or 8 minutes in various electric fields.  The team
then analysed the treated wine for chemical changes that might
alter its "mouth feel" and quality, and passed it to a panel of
12 experienced wine tasters who assessed it in a blind tasting
(Innovative Food Science and Emerging Technologies , vol 9, p
463 ).

The results were striking. With the gentlest treatment, the
harsh, astringent wine grew softer. Longer exposure saw some of
the hallmarks of ageing emerge-  a more mature "nose",
better balance and greater complexity. The improvements reached
their peak after 3minutes at 600 volts per centimetre: this left
the wine well balanced and harmonious, with a nose of an aged
wine and, importantly, still recognisably a cabernet sauvignon.

Two other good things happened: the breakdown of proteins
produced free amino acids that contribute to taste and there was
a noticeable reduction in the levels of aldehydes, which are
responsible for "off" flavours. You can have too much of a good
thing, though. Upping the voltage and applying it for longer
brought new and unwanted changes, including the generation of
new undesirable aldehydes. Zap it too much and the result, the
panel found, was worse than the untreated original.

Although Zeng cannot yet explain how exposure to an electric
field alters the wine's chemistry, his results show that under
the right conditions the technique can accelerate some aspects
of the ageing process. "Not only can it shorten a wine's normal
storage time, it can also improve some lower-quality wine," he
says. "It works just as well with other grape varieties such as
merlot and shiraz." Five Chinese wineries have begun trials.

A quick blast with an electric field can improve lower-quality
wine and shorten storage time.

Sadly for wine drinkers feeling the pinch, there's no immediate
prospect that you can try this for yourself. "I have thought of
designing a set of equipment for use at home," admits Zheng
"...but not yet."

---

[**http://www.sciencedirect.com**](http://www.sciencedirect.com)

***Innovative Food Science & Emerging Technologies --*** Volume
9,
Issue 4, October 2008, Pages 463-468

**The Effects of AC Electric Field on Wine
Maturation**

**Xin An Zenga**, Corresponding Author Contact Information,
E-mail The Corresponding Author, **Shu Juan Yua, Lu Zhangb**
and **Xiao Dong Chenc**

a College of Light Industry and Food Sciences, South China
University of Technology, Guangzhou 510640, PR China

b Department of Chemical and Materials, Faculty of Engineering,
The University of Auckland, New Zealand

c Departement of Chemical Engineering, Faculty of Engineering,
Monash University, Clayton, VIC 3800, Australia

**Abstract --** A pilot plant scale innovative technique
applying AC high voltage electric field to accelerate wine aging
of Young Cabernet Sauvignon is reported in this paper. The
design principles, equipment configuration and its effect on
wine taste and flavour are presented. Results from a sensory
evaluation group demonstrated that there were various effects on
the wine quality under different conditions, some positive while
others negative. An optimum treatment, with electric field 600
V/cm and treatment time 3 min, was identified to accelerate wine
aging, which made the harsh and pungent raw wine become
harmonious and dainty. HPLC and GC/MS combined with routine
chemical analysis methods were used to identify the differences
between the treated and untreated samples. It was found that the
contents of higher alcohols as well as aldehydes in volatile
compounds decreased to a large number, meanwhile, the contents
of esters and free amino acids slightly increased while others
remained unchanged through all treatments. The results of this
study show that the technology of accelerating wine aging by
high voltage electric field is a feasible method to shorten wine
maturing process times and to improve the quality of a young
wine, if favourable process conditions are chosen.   
Industrial relevance

The application of physical treatment methods other than heat,
such as electric field, magnetic field, ultrasonic wave and
microwave, etc., for green processing of foods, is becoming
popular. AC electric current is of continuous wave form, thus
being seldom used in food processing. However, numerous previous
studies about the effect and mechanisms of accelerating wine
aging with high voltage AC electric field have been conducted in
the Laboratory of South China University of Technology. This
manuscript presents the effect of high intensity AC electric
field on young wines physicochemical properties and sensory
quality. The results presented in this paper show that it is a
promising and novel technology to shorten the young wines aging
period. Recently, a few of the Chinese winery companies have
already started to set up the plant scale equipment.

Corresponding Author -- Contact Information :   
Tel.: +86 20 87113668; fax: +86 20 87112668

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**XIN AN ( "ANDY" ) ZENG**

![](zheng.jpg)

Birth date: Oct. 1972   
Birth place: Hu Nan province, China   
Blood type: O

**Tel: 0086-20-87113668 (o)**

*Address:*

**Room 13339, Building 13**   
**Campus of South China University of Technology**   
**No.381, Wushan Road**   
**Guangzhou City**   
**P.R.China, 510640**

**e-mail: xazengscut.edu.cn**

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**CN1623448**   
**Prepolarized Pulse Electric Field
Disinfectant Method and its Equipment**

Inventor:  ZENG XINAN [CN] ; FU XIONG   
Applicant:  UNIV SOUTH CHINA TECH   
2005-06-08   
Inventor(s):  ZENG XINAN [CN]; FU XIONG [CN]; YU SHUJUAN
[CN]   
Applicant(s):  UNIV SOUTH CHINA TECH [CN]   
Classification:  - international:  A23L3/00; A23L3/32;
A23L3/00; A23L3/32; (IPC1-7): A23L3/32; A23L3/00   
Also published as:  CN1285291  (C)

![](cn1623.jpg)![](cn1623b.jpg)

**Abstract** --  A prepolaried pulsive electric field
method for sterilizing material includes such steps as
prepolarizing the raw material in an electrostatic field, and
sterilizing in a high-voltage pulsive electric field. Its
equipment is composed of raw material tank, pump, vacuum
degassing unit, high-voltage electric pulse generator, electric
pulse treating chamber, and prepolarizing unit. Its advantage is
high effect to kill bacteria and deactivate enzyme.

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**CN1635100**   
**Process for Accelerating Ageing of Brandy
Oak by Electromagnetic Field**

Inventor:  ZENG XIN AN [CN] ; GENG YUHUAN   
Applicant:  UNIV SOUTH CHINA TECH   
2005-07-06   
Also published as: CN1303199  (C)

![](cn1635.jpg)

**Abstract** --  The invention discloses a method for
accelerating ageing of brandy oak by electromagnetic field,
which mainly consists of placing the brandy cask into 10-30KV/m
electromagnetic fields for aging treatment, the apparatus
comprises an electromagnetic field regulator assembly and an
ageing treatment charber, wherein the electromagnetic field
regulator assembly comprises an automatic alarm circuit breaking
component, a voltage-stabilizer, a frequency regulator and a
voltage adjusting magnifier, the ageing treatment chamber
comprises an electrode plate, an insulating frame and a cask,
the cask is arranged between the electrode plates.

**[ Shades of [Leon SPRINK :
Space Activator](../sprink/sprink.htm) ! ]**

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**CN1411871**   
**Processor of Pulse Electric Field
Sterilizing Equipment**

Inventor:  ZENG XIN AN [CN] ; LI GUOJI   
Applicant:  HUA NAN UNIV OF SCIENCE & ENGI   
2003-04-23   
Also published as:  CN1194765  (C)

**Abstract** --  The present invention relates to a
treatment device of equipment for sterilizing liquid products in
the fields of food, biological, pharmaceutical and chemical
industries by adopting high-intensity pulse electric field. Said
treatment device is formed from material-treating cavity, two
electrodes mounted in the material-treating cavity, inlet and
outlet, in which said material-treating cavity is vacuum cavity,
and can obtain good sterilizing effect.

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**CN1354018**   
**High-voltage pulse electric field
sterilization method and its equipment**

Inventor:  ZENG XIN AN [CN] ; FU XIONG   
Applicant:  HUA NAN UNIV OF SCIENCE & ENGI   
2002-06-19   
Also published as:  CN1174691  (C)

![](cn1354.jpg)

**Abstract** -- The present invention relates to a
sterilization method by using high-voltage impulse electric
field. The sterilization equipment designed according to said
method is formed from high-voltage impulseelectricity generation
device, parameter measurement, display and regulation and
control device and high-voltage impulse electric field treatment
chamber. In the course of sterilization it does not utilize
thermal power to kill microbe, but makes the material to be
sterilized pass through the high-voltage impulse electromagnetic
field. The microbe can be acted by strong electromagnetic field
force in a short time, its cell structure can be broken by
potential difference between cell membranes so as to make
thallus die.

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**CN2379480**   
**Pipeline Type High Voltage Electro-Magnetic
Field Quick Aging-Promoting Equipment for Wine**

Inventor:  ZENG XIN-AN [CN] ; LI GUOJI   
Applicant:  HUA NAN SCIENCE & ENGINEERING   
2000-05-24

![](cn2379a.jpg)

![](cn2379b.jpg)

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**US5690978**

**HIGH VOLTAGE PULSED ELECTRIC FIELD TREATMENT CHAMBERS FOR
THE PRESERVATION OF LIQUID FOOD PRODUCTS**

1998-04-09   
Inventor(s):  YIN YONGGUANG; ZHANG QINGHUA HOWARD; SASTRY
SUDHIR KARTIKEYA   
Applicant(s):  OHIO STATE RES FOUND   
Classification:  - international:  A23L3/00; A23L3/26;
A23L3/32; A23L3/00; A23L3/26; A23L3/32; (IPC1-7): A23L3/00;
A23L3/26; A23L3/32 - European:  A23L3/00; A23L3/26;
A23L3/32   
Also published as  **WO9814074  //**
AU4605697 //  EP1028635  // CA2267196   
Cited documents:  US4723483 (A)  US4838154 (A) 
US5235905 (A)

**Abstract --** A pulsed electric field treatment
device for the sterilization and preservation of pumpable food
products having at least two electrodes (201, 203) and an
insulator (202) and particularly suited for the inactivation of
vegetative and bacterial spore micro-organisms. Each electrode
includes an electrode flow chamber (207, 208) for making
electrical contact with the pumpable food product and for
allowing the pumpable food product to flow through the treatment
devices. The insulator (202) is situated between the electrodes
(201, 203) and includes an insulator flow chamber (206)
positioned between the electrode flow chambers (207, 208) and
provides for the flow of pumpable food product from one
electrode flow chamber to the other. A high voltage pulse
generator (107) applies a high voltage signal of variable
voltage, frequency and pulse duration to the electrodes.; The
electrode and insulator flow chambers may employ a variety of
sectional and cross-sectional geometries including tubular,
cylindrical, rectangular, elliptical and non-uniform design.

Current U.S. Class:   426/237 ; 426/521; 99/451;
99/483; 99/DIG.14   
Current International Class:  A23L 3/00 (20060101); A23L
3/32 (20060101); A23L 3/26 (20060101); A23L 003/00 (); A23L
003/26 (); A23L 003/32 ()   
Field of Search:  99/451,483,DIG.14,516,536,358
426/234,237,238,521,410,241,407,247,248 422/22-24 219/700,735
392/338,497

**References Cited [Referenced By]**   
**U.S. Patent Documents**

3876373 April 1975 Glyptis   
4524079 June 1985 Hofmann   
4695472 September 1987 Dunn et al.   
4838154 June 1989 Dunn et al.   
5048404 September 1991 Bushnell et al.   
5235905 August 1993 Bushnell et al.   
5250160 October 1993 Oksman et al.   
5282940 February 1994 Griffis et al.   
5290583 March 1994 Reznik et al.   
5393541 February 1995 Bushnell et al.   
5415882 May 1995 Knipper et al.   
5447733 September 1995 Bushnell et al.   
5514391 May 1996 Bushnell et al.   
5527105 June 1996 Riach, Jr.   
5534278 July 1996 DeRuyter et al.   
5549041 August 1996 Zhang et al.   
5562024 October 1996 Polny, Jr.   
5603972 February 1997 McFarland   
5607710 March 1997 DeRuyter et al.   
5630360 May 1997 Polny, Jr.

**Foreign Patent Documents**

 2 513 087  Sep., 1981  FR   
 3-98565  Apr., 1991  JP

**Other References**

"Engineering Aspects of Pulsed Electric Field Pasteurization,"
Zhang, Qinghua, et al., Journal of Food Engineering, 25:261-281,
1994. .   
"Inactivation of E. coli and S. cerevisiae by Pulsed Electric
Fields Under Controlled Temperature Conditions," Zhang, Q., et
al., 1994 American Society of Agricultural Engineers, vol.
37(2);581-587. .   
"Inactivation of Microorganisms in a Semisolid Model Food Using
High Voltage Pulsed Electric Fields," Zhang, Qinghua, et al.,
Food Science & Technology (lwt), 1994, 2(6):538..

**Description**

**FIELD OF THE INVENTION**

The present invention relates generally to food preservation
systems and methods and, more specifically, to a pulse electric
field treatment device for inactivating bacteria and
microorganisms found in liquid food products.

**BACKGROUND OF THE INVENTION**

The preservation of food products is an important industrial
and commercial activity and is primarily based on the
inactivation, or destruction, of microorganisms in the food
product. Existing methods of food preservation include the use
of salts (i.e. sodium chloride), dehydration, heat
pasteurization and freezing to inactivate microorganisms present
in the food product. However, many of these processes affect the
color, texture, flavor and taste of the foods preserved. For
example, heat pasteurization can cause thermal damage to the
food product and adversely affect its taste, flavor and nutrient
content. As a result of this and other disadvantages,
researchers have strived to develop non-thermal food
sterilization techniques.

One such non-thermal food sterilization technique is known as
Pulsed Electric Field (hereinafter PEF) treatment. Generally, in
PEF treatment, an electric field voltage is applied across two
electrodes where food material is between the electrodes.
Because most liquid food products are primarily composed of
water and nutrients such as proteins, vitamins, triglycerides
and minerals, a corresponding electric field is induced in the
food product and weakens the bacteria's cell structure. The
bactericidal effect caused by PEF treatment is best explained by
what is known as the Dielectric Rupture Theory.

According to the Dielectric Rupture Theory, PEF treatment
reduces the activity of bacteria and other microorganisms by
damaging the bacterial or microorganism cell structure. The
applied electric field induces an electric potential across the
membrane of a living cell. This electric potential, in turn
causes an electrostatic charge separation in the cell membrane
based on the polar nature of the cell membrane molecules. When
this electric potential exceeds a critical value, pores form in
weak areas of the cell membrane. When the critical value is
exceeded by a large margin, the pore formation and cell membrane
damage have a lethal effect on the bacteria or microorganism.
However, the lethal effect of PEF treatment is dependent on many
factors including, inter alia, the strength and length of time
the electric field is applied, treatment temperature and the
species of bacteria and/or microorganism to be inactivated. For
further information on the engineering aspects of PEF treatment,
see Zhang, Q., G. V. Barbosa-Canovas and B. G. Swanson,
Engineering Aspects of Pulsed Electric Field Pasteurization,
Journal of Food Engineering, Vol. 25 pp. 261-281 (1994).

In particular, the lethal effect of PEF treatment on bacterial
spores has heretofore been limited. The limited results are due
in part to the bacterial spore's rigid structure and ability to
resist unfavorable environmental conditions. Therefore, current
PEF treatment methods and devices for the inactivation of
bacterial spores have met with only limited success.

**SUMMARY OF THE INVENTION**

According to the present invention, a pulse electric field
(PEF) treatment device is provided for preserving and
sterilizing a liquid food product. The PEF treatment device
includes a first and second electrode for supplying an electric
field to the liquid food product. Each electrode includes an
electrode flow chamber for accepting the flow of the liquid food
product and for making electrical contact with the liquid food
product. The PEF treatment device also includes at least one
insulator positioned between the first and second electrodes and
for electrically insulating the first and second electrodes from
each other. The electrical insulator includes an insulator flow
chamber for accepting the flow of liquid food product from the
electrode flow chamber of the first electrode and the electrode
flow chamber of the second electrode. The electrode flow chamber
of the first electrode includes an inlet aperture and an outlet
aperture. Similarly, the electrode flow chamber of the second
electrode includes an inlet aperture and an outlet aperture. The
insulator flow chamber also includes an inlet aperture and an
outlet aperture. The insulator flow chamber and the electrode
flow chambers are configured to comprise a single tubular flow
chamber for accepting the flow of the liquid food product
through the PEF treatment device. The insulator flow chamber
inlet aperture and the first electrode flow chamber outlet
aperture are positioned adjacent to each other and have
substantially similar cross-sectional geometry. Similarly, the
insulator flow chamber outlet aperture and the second electrode
flow chamber inlet aperture are positioned adjacent to each
other and have substantially similar cross-sectional geometry.

The present invention also provides a PEF treatment system for
preserving and sterilizing a liquid food product. The system
includes a high voltage pulse generator for supplying a pulsed
electric field, a PEF liquid product treatment device for
subjecting the liquid product to the pulsed electric field, a
balance tank for storing the liquid food product to be treated,
a de-oxygenator device for removing oxygen and other gases from
the liquid food product, a pulseless pump for providing a
continuous flow of liquid food product in the treatment system,
at least one heat exchanger for regulating the temperature of
the liquid food product, and an aseptic packaging device for
packaging the liquid food product. The PEF liquid product
treatment device is in circuit communication with the high
voltage pulse generator and includes first and second electrodes
for supplying an electric field to the liquid food product and
an insulator for electrically insulating the plurality of
electrodes from each other. Each electrode includes an electrode
flow chamber for accepting the flow of the liquid product and
for making electrical contact with the liquid product. The
insulator includes an insulator flow chamber for accepting the
flow of liquid food product and is positioned between the
electrode flow chambers. The insulator flow chamber and the
electrode flow chambers are configured so that a single tubular
flow chamber for accepting the flow of liquid food product is
formed thereby and each have inlet and outlet apertures of
substantially similar cross-sectional geometry and adjacent to
each other.

The present invention further provides a PEF treatment device
having first and second electrodes for supplying an electric
field to a liquid product, at least one insulator for
electrically insulating the first and second electrodes from
each other and an insertion member for providing electrical
contact to the liquid product. Each electrode includes an
electrode flow chamber for accepting the flow of liquid product
and each insulator includes an insulator flow chamber for
allowing the liquid product to flow from and to the electrode
flow chambers. The electrode flow chambers and the insulation
flow chamber are configured and positioned so as to form a
single flow chamber for accepting the flow of the liquid
product. The flow chambers each have inlet and outlet apertures
of substantially similar apertures and are adjacent to each
other. The insertion member is positioned within the electrode
and insulator flow chambers and includes a plurality of
conducting members and at least one insulator member. The
plurality of conducting members provide electrical contact to
the liquid product and the at least one insulator member
provides electrical insulation between the plurality of
conducting members. The insertion member includes a cylindrical
body that is concentrically located within the single flow
chamber that is comprised by the electrode and insulator flow
chambers.

The present invention further provides a method of inducing a
pulsed electric field in a liquid product for the inactivation
of bacterial spores. The method includes the steps of pumping
the liquid product through a treatment device so as to create a
liquid product flow in the treatment device, generating a
plurality of pulsed electric fields, and inducing the plurality
of pulsed electric fields in the liquid product wherein the
induced, pulsed electric field vector direction is parallel to
the liquid product flow. Furthermore, the step of generating a
plurality of pulsed electric fields includes the step of
generating a pulsed electric field with a frequency range of 500
Hz to 20 kHz and an electric field range of 15 kV/cm to 160
kV/cm. The method further includes the step of regulating the
liquid product temperature in the range of 31.degree. to
36.degree. C. Additionally, the step of generating a pulsed
electric field includes a step of generating a pulsed electric
field with a pulse length of 1 to 20 microseconds.

It is therefore an advantage of the present invention to
provide a PEF treatment device that includes at least one
tubular flow treatment chamber.

It is a further advantage of this invention to provide a PEF
system for the preservation of liquid food products that
includes a pulseless pump for providing a constant velocity of
liquid product to ensure uniform treatment dosage.

It is further another advantage of this invention to provide a
PEF system, device and method for the inactivation of bacterial
spores in addition to vegetative microorganisms, thereby making
the system, device and method suitable for the sterilization of
food, nutriceutic, cosmetic and pharmaceutical products.

**BRIEF DESCRIPTION OF THE DRAWINGS**

In the accompanying drawings which are incorporated in and
constitute a part of the specification, embodiments of the
invention are illustrated, which, together with a general
description of the invention given above, and the detailed
description given below, serve to example the principles of this
invention.

**FIG. 1** is a schematic illustration of the PEF system of
the present invention for extending the shelf life of
perishable, pumpable liquid products utilizing a pulsed electric
field (PEF) treatment device.

![](us569a.jpg)

**FIG. 2A** is a cross-sectional side view of a first
embodiment of a PEF treatment device having cylindrical
electrodes and a cylindrical insulator.

![](us569b.jpg)

**FIG. 2B** is a sectional view taken along section line
2B--2B of FIG. 2A.

![](us569c.jpg)

**FIG. 3A** is a cross-sectional side view of a second
embodiment of a PEF treatment device having non-cylindrical
electrodes and a non-cylindrical insulator.

![](us569-3.jpg)

**FIG. 3B** is a sectional view taken along section line
3B--3B of FIG. 3A.

![](us569-3b.jpg)

**FIG. 4** is a cross-sectional side view of a third
embodiment of a PEF treatment device having tapered electrode
flow chambers.

![](us569-4.jpg)

**FIG. 5** is a cross-sectional side view of a fourth
embodiment of a PEF treatment device having three electrodes and
two insulators.

![](us569-5.jpg)

**FIG. 6** is a cross-sectional side view of a fifth
embodiment of a PEF treatment device having two electrodes of
different cross-sectional geometry.

![](us569-6.jpg)

**FIG. 7** is a cross-sectional side view of a sixth
embodiment of a PEF treatment device.

![](us569-7.jpg)

**FIG. 8A** is a cross-sectional side view of a seventh
embodiment of a PEF treatment device having conducting and
insulating members concentrically located within flow chambers.

**FIG. 8B** is a sectional view taken along section line
8B--8B of FIG. 8A,

![](us569-8.jpg)![](us569-8b.jpg)

**FIG. 9** is a graph illustrating the effect of pulse
duration time on the inactivation of bacterial spores at the
same energy input.

![](us569-9.jpg)

**FIG. 10** is a graph illustrating the effect of bacterial
spore inactivation as the pulsed electric field frequency is
varied,

![](us569-10.jpg)

**FIG. 11** is a graph illustrating the effect of
temperature variation on bacterial spore inactivation,

![](us569-11.jpg)

**FIG. 12** is a graph illustrating the effect on bacterial
spore inactivation in two treatment mediums as the treatment
time is varied,

![](us569-12.jpg)

**FIG. 13** is a bar graph illustrating the effect on
bacterial spore inactivation at different electric field
intensities as the treatment time is varied,

![](us569-13.jpg)

**DETAILED DESCRIPTION OF ILLUSTRATED EMBODIMENT**

**PEF Treatment System Structure**

A pulsed electric field (PEF) treatment system is illustrated
in FIG. 1. The system includes a balance tank 101, a
de-oxygenator 102, a pulseless pump 104, heat exchangers 105A, B
and C, PEF treatment devices 106A, B and C, a high voltage pulse
generator 107 and an aseptic packaging device 108. The balance
tank 101 holds the liquid product to be treated by the PEF
system. The liquid product may be a wide range of products,
including edible food products having a viscosity or extrusion
capacity such that the food product may be forced into a flow
through a treatment chamber. Other liquid products include
extrudible products such as doughs or meat emulsions, fluid
products such as beverages, fluid dairy products, gravies,
sauces and soups, and food particulate containing food slurries
such as stews, and food particulate containing soups, and cooked
or uncooked vegetable or grain slurries. Moreover, the liquid
food product may include nutriceutic, cosmetics and
pharmaceutical products. The balance tank 101 is in physical
communication with the de-oxygenator 102. The de-oxygenator 102
serves to remove dissolved gasses and/or product bubbles which
might adversely affect the development of a substantially
uniform electric field in the liquid product as it is treated in
PEF treatment devices 106A, B and C.

The pulseless pump 104 is in physical communication with the
de-oxygenator unit 102. The pulseless pump 104 provides the PEF
treatment system with a flow of liquid product at a constant
velocity to ensure uniform treatment. The pulseless pump 104 is
further in physical communication with heat exchanger 105A and
PEF treatment device 106A.

The heat exchangers 105A, B and C are in physical communication
with PEF treatment devices 106A, B and C as shown in FIG. 1.
While FIG. 1 illustrates the use of three heat exchangers
interdigitized between three PEF treatment devices, different
combinations involving more or less heat exchangers and PEF
treatment devices are possible. For example, the PEF treatment
system may include only one heat exchanger and one PEF treatment
device. Generally, the number of heat exchangers and the number
of PEF treatment devices will depend on the system design and
treatment objectives. The heat exchangers 105A, B and C
primarily serve as temperature regulators for the PEF treatment
system. As shown in FIG. 1, the liquid product's temperature is
first regulated by a heat exchanger before entering a PEF
treatment device for sterilization or bacterial inactivation.

Still referring to FIG. 1, the high voltage pulse generator 107
is in circuit communication with each PEF treatment device. The
high voltage pulse generator 107 provides PEF treatment devices
with a high voltage wave form having variable voltage levels,
frequency ranges and pulse duration times.

The last PEF treatment device (106C) is in physical
communication with the aseptic packaging device 108. The aseptic
packaging device 108 serves to aseptically pack and seal the
liquid product in a sterile environment. One such commercially
available aseptic packaging device is the Benco LaborPack/2
Aseptic Packaging Machine.

**PEF Treatment Device Structure**

Referring now to FIG. 2A, a cross-sectional side view of an
embodiment of a pulsed electric field treatment device 200 is
shown. The PEF treatment device 200 includes a first electrode
201 and a second electrode 203 and an insulator 202. First
electrode 201 includes an electrode flow chamber 207 for making
electrical contact with the liquid product and for allowing the
liquid product to flow through the PEF treatment device 200.
Similarly, electrode 203 includes an electrode flow chamber 208
for making electrical contact with the food product and for
allowing the food product to flow out of the PEF treatment
device 200. The electrode flow chambers 207 and 208 each include
inlet and outlet apertures at 213 and 209, and at 211 and 215
respectively.

The insulator 202 is in physical communication with the first
electrode 201 and the second electrode 203. The insulator 202
includes an insulator flow chamber 206 for allowing the flow of
liquid product from the first electrode 201 to the second
electrode 203. The insulator flow chamber 206 is in physical
communication with the first electrode flow chamber 207 and the
second electrode flow chamber 208 and includes an inlet and
outlet aperture at 209 and 211 respectively. As shown in FIG.
2A, the first electrode 201, second electrode 203, insulator
202, first electrode flow chamber 207, insulator flow chamber
206, and second electrode flow chamber 208 are formed and
configured such that the electrode flow chambers 207 and 208 and
the insulator flow chamber 206 form a single tubular flow
chamber through the PEF treatment device. The insulator flow
chamber inlet aperture and the first electrode flow chamber
outlet aperture are positioned adjacent to each other and have
substantially similar cross- sectional geometry. Similarly, the
insulator flow chamber outlet aperture and the second electrode
flow chamber inlet aperture are positioned adjacent to each
other and have substantially similar cross-sectional geometry.
The phrase "substantially similar" is hereinafter used to
express the concept that one may depart from the overall inlet
and outlet aperture dimensions and shape and still maintain an
overall degree so similarity in geometry and/or functionality.
For example, inlet and outlet physical dimensions and shapes
need not be identical, that is, the insulator flow chamber inlet
or outlet apertures may be slightly larger or smaller than the
electrode flow chamber outlet or inlet apertures, or vice-versa.
Moreover, the electrode flow chamber inlet or outlet aperture
may be elliptical while the insulator flow chamber outlet or
inlet aperture may be circular, or vice-versa. Therefore, it
should be apparent that an electrode flow chamber's inlet and/or
outlet apertures and the insulator flow chamber's inlet and/or
outlet apertures may have different dimensions and shapes and
still maintain substantially similar cross-sectional geometries.

Referring now to FIG. 2B, a sectional view taken along a
section line 2B--2B of FIG. 2A of PEF treatment device 200 is
shown. The insulator 202 further includes an outer cylindrical
insulator surface 214 and an inner cylindrical insulator surface
at boundary 210. The electrode 201 includes an outer cylindrical
electrode surface at boundary 210 that is in physical
communication with the inner cylindrical insulator surface, also
located at boundary 210. The electrode 201 further includes an
inner cylindrical electrode surface 212 that forms the electrode
flow chamber 207.

Referring now to FIG. 3A, a cross-sectional side view of a
second embodiment of a pulsed electric field treatment device
300 is shown. As evident from FIG. 2A, PEF treatment device 300
and PEF treatment device 200 have substantially similar
cross-sectional side views. In particular, the PEF treatment
device 300 includes a first electrode 301, a second electrode
303 and an insulator 302. First electrode 301 includes an
electrode flow chamber 307 for making electrical contact with
the liquid product and for allowing the liquid product to flow
through the PEF treatment device 300. Similarly, the electrode
303 includes an electrode flow chamber 308 for making electrical
contact with the food product and for allowing the food product
to flow out of the PEF treatment device 300. The electrode flow
chambers 307 and 308 each include inlet and outlet apertures.

The insulator 302 is in physical communication with the first
electrode 301 and the second electrode 303. The insulator 302
includes an insulator flow chamber 306 for allowing the flow of
liquid product from the first electrode 301 to the second
electrode 303. The insulator flow chamber 306 is in physical
communication with the first electrode flow chamber 307 and the
second electrode flow chamber 308 and includes an inlet and an
outlet aperture. As shown in FIG. 3A, the first electrode 301,
second electrode 303, insulator 302, first electrode flow
chamber 307, insulator flow chamber 306, and second electrode
chamber 308 are formed and configured such that the electrode
flow chambers 307 and 308 and the insulator flow chamber 306
form a single tubular flow chamber through the PEF treatment
device 300. The insulator flow chamber inlet aperture and the
first electrode flow chamber outlet aperture are positioned
adjacent to each other and have substantially similar
cross-sectional geometry. Similarly, the insulator flow chamber
outlet aperture and the second electrode flow chamber inlet
aperture are positioned adjacent to each other and have
substantially similar cross-sectional geometry. In all
embodiments hereinafter, the above insulator flow chamber inlet
and outlet aperture configuration with electrode flow chamber
inlet and outlet apertures shall be generally applicable.

Referring now to FIG. 3B, a sectional view taken along a
section line 3B--3B of FIG. 3A of PEF treatment device 300 is
shown. The insulator 302 further includes an outer rectangular
surface 314 and an inner rectangular surface having rounded ends
at boundary 310. The first electrode 301 also includes an outer
rectangular surface with rounded ends at boundary 310 and
further is in physical communication with the inner rectangular
insulator surface, also located at boundary 310. The first
electrode 301 further includes an inner rectangular surface 312
with rounded ends 316 and 318. The inner rectangular surface 312
forms the electrode flow chamber 307. It should be noted that
from FIGS. 2B and 3B, the sectional geometry of a PEF treatment
device may take on various geometries based on design factors
such as the nature of the liquid product (viscosity,
particulate, electrical resistivity, etc.), liquid product flow
rate, applied electric field strength, etc. Therefore, the PEF
treatment device cross-sectional geometries may range from
tubular or cylindrical cross-sections with tubular or
cylindrical flow chambers, as shown in FIG. 2B, to rectangular
cross-sections with rectangular flow chambers, as shown in FIG.
3B. Other possible cross-sectional geometries for the PEF
treatment device and the flow chambers include uniform and
non-uniform geometries and various elliptical geometries.

Illustrated in FIG. 4 is a cross-sectional side view of a third
embodiment of a pulsed electric field treatment device 400,
having tapered electrode flow chambers 407 and 408. The PEF
treatment device 400 includes a first electrode 401 and a second
electrode 403, and an insulator 402. The first electrode 401
includes an electrode flow chamber 407 for making electrical
contact with the liquid product and for allowing the liquid
product to flow through the PEF treatment device 400. The
electrode flow chamber 407 includes tapered surfaces 424 and 426
and inlet and outlet apertures. Similarly, electrode 403
includes an electrode flow chamber 408 for making electrical
contact with the liquid product and for allowing the liquid
product to flow out of the PEF treatment device 400. Moreover,
electrode flow chamber 408 includes tapered surfaces 420 and 422
and inlet and outlet apertures. The tapered surfaces 424 and 426
may be elements of various cross-sectional designs of the
electrode flow chamber 407. Similarly, tapered surfaces 420 and
422 may be elements of various cross-sectional designs of
electrode flow chamber 408. For example, electrode flow chambers
407 and 408 may include conical sectional geometries which
include the tapered surfaces 424, 426, 420 and 422. Furthermore,
the electrode flow chambers 407 and 408 may have rectangular or
elliptical sectional geometries with decreasing areas that
include tapered surfaces 424, 426, 420 and 422. Consequently,
electrode flow chambers 407 and 408 can have any geometrically
tapered sectional or cross-sectional geometry that is suitable
for the system design specifications.

Illustrated in FIG. 5 is a cross-sectional side view of a
fourth embodiment of a pulsed electric field treatment device
500, having three electrodes and two insulators. The PEF
treatment device 500 includes a first electrode 501, a second
electrode 503, a third electrode 505, a first insulator 502 and
a second insulator 504. The first electrode 501 includes an
electrode flow chamber 507 for making electrical contact with
the liquid product and for allowing the liquid product to flow
through the PEF treatment device 500. Similarly, second and
third electrodes 503 and 505 include electrode flow chambers 508
and 510 for making electrical contact with the liquid product
and for allowing the liquid product to flow through and out of
the PEF treatment device 500. The first insulator 502 is in
physical communication with the first electrode 501 and second
electrode 503. The first insulator 502 includes an insulator
flow chamber 506 for allowing the flow of liquid product from
the first electrode 501 to the second electrode 503. Similarly,
the second insulator 504 is in physical communication with the
second electrode 503 and the third electrode 505. The second
insulator 504 also includes an insulator flow chamber 509 for
allowing the flow of liquid product from the second electrode
503 to the third electrode 505. The insulator flow chamber 506
is in physical communication with the first electrode flow
chamber 507 and the second electrode flow chamber 508 and is
positioned therebetween. Similarly, insulator flow chamber 509
is in physical communication with the second electrode flow
chamber 508 and the third electrode flow chamber 510, and is
positioned therebetween. As shown in FIG. 5, the electrode flow
chambers 507, 508 and 510, and insulator flow chambers 506 and
509 are formed and configured such that the electrode flow
chambers and the insulator flow chambers form a single flow
chamber through the PEF treatment device 500.

Illustrated in FIG. 6 is a cross-sectional side view of a fifth
embodiment of a pulsed electrical field treatment device 600,
having two electrodes of different cross-sectional geometries.
The PEF treatment device 600 includes a first electrode 601 and
a second electrode 603 and an insulator 602. The first electrode
601 includes a tapered electrode flow chamber 607 for making
electrical contact with the liquid product and for allowing the
liquid product to flow through the PEF treatment device 600. The
tapered electrode flow chamber 607 includes tapered surfaces 624
and 626. As was described for electrode flow chamber 407 of FIG.
4, electrode flow chamber 607 can employ a variety of sectional
and cross-sectional geometries based on the design
specifications of the PEF treatment system.

The insulator 602 is in physical communication with the first
electrode 601 and the second electrode 603. The insulator 602
includes an insulator flow chamber 606 for allowing the flow of
liquid product from the first electrode 601 to the second
electrode 603. The insulator flow chamber 606 is in physical
communication with the first electrode flow chamber 607 and the
second electrode flow chamber 608 and is positioned
therebetween. As shown in FIG. 6, the electrode flow chambers
607 and 608 and the insulator flow chamber 606 are configured
such that a single flow chamber is formed through the PEF
treatment device 600.

Illustrated in FIG. 7 is a cross-sectional side view of a sixth
embodiment of a pulsed electric field treatment device 700
having removable components. The PEF treatment device 700
includes a first and second electrode 701 and 703. The first
electrode 701 includes an electrode flow chamber 707 for making
electrical contact with the liquid product and for allowing the
flow of liquid product into PEF treatment device 700. Similarly,
the second electrode 703 includes an electrode flow chamber 708
for making electrical contact with the liquid product and from
allowing the flow of liquid product to exit the PEF treatment
device 700. The PEF treatment device 700 further includes a
first insulator 702 and a second insulator 710. The first and
second insulators 702 and 710 are in physical communication with
each other and are connected via threading surfaces 718 and 720.
The first insulator 702 includes inner threads, and the second
insulator 710 includes outer threads such that the insulators
702 and 710 may be tightly threaded together.

Still referring to FIG. 7, the PEF treatment device 700 further
includes an insulation cushion 712 and a first and second
insulation washer 714 and 716. The insulation cushion 712 is in
physical communication with the first and second electrodes 701
and 703, the first and second insulators 702 and 710, and the
first and second insulation washers 714 and 716. Furthermore,
insulation cushion 712 includes an insulator flow chamber 706
for allowing the flow of liquid product from the first electrode
701 to the second electrode 703. The configuration of the
various components shown in FIG. 7 of PEF treatment device 700
are all concentric so that PEF treatment device 700 may be
quickly assembled or disassembled via a twisting or turning
motion due to the threaded interconnection of first insulator
702 and second insulator 710. This provides PEF treatment device
700 with the major advantages of low cost and easy maintenance.
Additionally, PEF treatment device 700 has the advantage of
allowing easy removal and replacement of the insulator cushion
712 and the insulator flow chamber 706.

Referring now to FIG. 8A, a cross-sectional side view of a
seventh embodiment of a pulsed electric field treatment device
800 is shown. The PEF treatment device 800 includes a first
electrode 801 and a second electrode 803 and a first insulator
802. First electrode 801 includes an electrode flow chamber 807
for making electrical contact with the liquid product and for
allowing the liquid product to flow through the PEF treatment
device 800. Similarly, electrode 803 includes an electrode flow
chamber 808 for making electrical contact with the food product
and for allowing the food product to flow out of the PEF
treatment device 800. The first electrode 801 is in physical
and/or circuit communication with a first conducting insert
member 810 and the second electrode 803 is, similarly, in
physical and/or circuit communication with a second conducting
insert member 811. The first and second conducting members 810
and 811 are concentric with first and second electrodes 801 and
803 and are concentrically located within electrode flow
chambers 807 and 808. Moreover, the first and second electrodes
801 and 803 may include an integrated construction that includes
insert members 810 and 811 respectively. In the illustrated
embodiment, the conducting insert members 810 and 811 have
rod-like geometries.

The insulator 802 is in physical communication with the first
electrode 801 and the second electrode 803. The insulator 802
includes an insulator flow chamber 806 for allowing the flow of
liquid product from the first electrode 801 to the second
electrode 803. The insulator flow chamber 806 is in physical
communication with the first electrode flow chamber 807 and the
second electrode flow chamber 808. An insulator insert member
809 is concentrically locted within insulator flow chamber 806
and is in physical communication with first and second
conducting insert members 810 and 811. As shown in FIG. 8A, the
first electrode flow chamber 807, insulator flow chamber 806,
second electrode flow chamber 808, conducting insert members 810
and 811 and insulating insert member 809 are formed and
configured such that the electrode flow chambers 807 and 808 and
the insulator flow chamber 806 form a single tubular flow
chamber through the PEF treatment device.

Referring now to FIG. 8B, a sectional view taken along a
section line 8B--8B of FIG. 8A of PEF treatment device 800 is
shown. The insulator 802 further includes an outer cylindrical
insulator surface 814 and an inner cylindrical insulator surface
at boundary 816. The electrode 801 includes an outer cylindrical
electrode surface at boundary 816 that is in physical
communication with the inner cylindrical insulator surface, also
located at boundary 816. The electrode 801 further includes an
inner cylindrical electrode surface 812 that forms the electrode
flow chamber 807. The first conducting insert member 810
includes an outer cylindrical member 818 and is concentrically
positioned within electrode flow chamber 807 to provide the flow
chambers 807, 806, and 808 with improved liquid product flow
characteristics (i.e. uniformity of liquid velocity), where such
improved liquid flow characteristics are desirable.

In all of the illustrated embodiments, the electrodes comprise
food-grade stainless steel. However, any other food-grade,
electrically conducting material may be substituted in the above
embodiments for the stainless steel. The various insulation
components of the presently described embodiments are comprised
of polycarbonate. However, the insulation components may be any
electrically insulating material such as ceramics, glass or
plastics.

**Operation of The PEF Treatment Devices**

To recall, the PEF treatment process applies an electric field
voltage across two electrodes where a liquid product, such as a
food product, exists between the electrodes. Because most liquid
food products are primarily composed of water and nutrients, a
corresponding electric field is induced in the food product. A
bactericidal effect, commonly called the Dielectric Rupture
Theory, arises due to this induced electric field in the liquid
food product. According to the Dielectric Rupture Theory, PEF
treatment reduces the activity of bacteria and other
micro-organisms by damaging the bacterial or micro-organism cell
structure. The applied electric field induces an electric
potential across the membrane of a living cell, which, in turn,
causes an electrostatic charge separation in the cell membrane
and results in pore formation in weak areas of the cell
membrane. The pore formation and cell membrane damage have a
lethal effect on the bacteria or micro-organism. The operation
of PEF treatment device 200 shown in FIGS. 2A and 2B will now be
presently described in detail with respect to the inactivation
of the Bacillus subtilis species of bacterial spore and with the
understanding that the operational description is equally
applicable to PEF treatment devices 300-800 shown in FIGS.
3A-8B.

Referring now to FIGS. 1, 2A and 2B, electrode 203 is in
circuit communication with a high voltage terminal of the high
voltage pulse generator 107 and electrode 201 is in circuit
communication with a ground network. Therefore, when a high
voltage pulse signal is applied across electrodes 203 and 201,
an electric field is formed in electrode flow chambers 207 and
208, and insulator flow chamber 206. Furthermore, due to the
physical configuration of electrodes 201 and 203 and insulator
202, the electric field strength will be strongest in insulator
flow chamber 206, and will have a vector direction pointing from
electrode 203 to electrode 201. Therefore, as liquid product
passes through electrode flow chamber 207 to insulator flow
chamber 206 and through electrode flow chamber 208, it is
subjected to an applied electric field that is concentrated in
insulator flow chamber 206. Accordingly, the liquid product in
insulator flow chamber 206 is subjected to the concentrated
applied electric field and because the liquid product is
primarily composed of water and nutrients, the concentrated
applied electric field will be present within the liquid
product. Therefore, the bactericidal effect of the PEF treatment
process of the present invention primarily occurs in the liquid
product flowing through insulator flow chamber 206.

Illustrated in FIG. 9 is a graph showing the effect of pulse
duration time on the inactivation of bacterial spores at a
constant applied electric field. More particularly, FIG. 9
illustrates the inactivation of bacterial spores with pulse
duration times of 1, 2, 4 and 6 microseconds and at frequencies
of 3,000, 1,500, 750 and 500 Hz, respectively, and at an applied
electric field strength of E=30 kV/cm and a temperature of
T=36.degree. C. FIG. 9 indicates that, with the same energy
input, when the pulse duration time is increased from 1 to 6
microseconds, the inactivation of spores increases. As shown in
FIG. 8, ninety-two percent (92%) of the spores were inactivated
with 6 microsecond pulse durations applied for 1,770
microseconds (i.e., 295 pulses). In FIG. 8, the frequency was
decreased in correspondence to the increased pulse duration
times so that a constant power input was maintained for
comparison purposes. As evident from FIG. 8, the frequency of
the applied electric field signal is a factor that affects the
inactivation rate of the bacterial spores.

Illustrated in FIG. 10 is a graph showing the effect of
bacterial spore inactivation as the pulsed electric field
frequency is varied. More particularly, FIG. 10 illustrates the
inactivation of bacterial spores at frequencies of 2,000, 3,000
and 4,000 Hz, with a pulse duration time of 3 microseconds and
an applied electric field strength of E=30 kV/cm at a
temperature of T=36.degree. C. Therefore, as the frequency
increases, the number of pulses and total treatment time that
the bacterial spores receive also increases. However, as shown
in FIG. 10, the inactivation rate of bacterial spores decreases
as the frequency increases. Since bacterial spores are hard, but
structurally fragile, FIG. 10 indicates that there is an optimal
PEF treatment frequency that may cause resonance of the
bacterial spore structure. This resonance causes a loosening of
the rigid bacterial spore structure such that the applied pulsed
electric field can, in effect, punch through the spore structure
and inactivate the spores.

Illustrated in FIG. 11 is a graph showing the effect of
temperature variation on bacterial spore inactivation.
Particularly, FIG. 11 illustrates the inactivation of bacterial
spores at different treatment temperatures with an applied
electric field strength of E=30 kV/cm at a frequency of f=1,500
Hz and a pulse duration time of .tau.=2 microseconds. The
inactivation of bacterial spores was tested at 20.degree.,
30.degree., 36.degree., 40 and 50.degree. C. The results shown
in FIG. 11 illustrate that there is an optimum temperature of
36.degree. C. for inactivating bacterial spores by PEF
treatment. FIG. 11 also illustrates that the inactivation rate
of bacterial spores increases with the total PEF treatment time
at 30.degree. and 36.degree. C. treatment temperatures, while
the inactivation rate does not exhibit significant change after
540 microseconds of total treatment time at 20.degree.,
40.degree. and 50.degree. C. treatment temperatures. These
results indicate that at the optimum treatment temperature, more
bacterial spores tend to germinate and to be inactivated as the
treatment time is extended.

Referring now to FIG. 12, a graph illustrating the effect on
bacterial spore inactivation in two treatment mediums as the
treatment time is varied is shown. FIG. 12 shows the
inactivation of bacterial spores in two treatment mediums: a
0.02% NaCl and a 0.02% NaCl+0.01% L-alanine. The PEF treatment
parameters for FIG. 12 were an applied electric field strength
of E=30 kV/cm, f=1,000 Hz, pulse duration time of .tau.=6
microseconds and a treatment temperature of T=36.degree. C.,
while two PEF treatment devices were used in series with an
insulator flow chamber of 2 mm. L-alanine is considered as a
germination agent for many strains of bacterial spores. As shown
in FIG. 12, the inactivation rate of bacterial spores in the
0.02% NaCl treatment medium supplemented with the 0.01%
L-alanine germination agent is higher than that of the 0.02%
NaCl treatment medium without any germination agent. Also as
shown in FIG. 12, for the first 120 microseconds of treatment
time, there is very little difference between the inactivation
rate of bacterial spores in the two treatment mediums. However,
when the treatment time reaches 300 microseconds, the
inactivation difference between the two mediums grows larger.
These results indicate that more bacterial spores tend to
germinate and be inactivated by PEF treatment as the treatment
time is increased. Moreover, the results of FIG. 12 are
consistent with the temperature results shown in FIG. 11.

Illustrated in FIG. 13 is a bar graph showing the effect on the
inactivation of bacterial spores at different applied electric
field intensities as the treatment time is varied. More
particularly, FIG. 13 shows the effect on bacterial spore
inactivation at three electric field levels: 30, 37 and 40
kV/cm, while maintaining the applied electric field frequency at
2,000 Hz and the pulse duration time at .tau.=3 microseconds and
at a treatment temperature of T=36.degree. C. FIG. 13 indicates
that as the electric field intensity increases, the inactivation
of bacterial spores increases. More particularly, after the
bacterial spores were exposed to an electric field strength of
40 kV/cm for 3.5 milliseconds, 98% of the bacterial spores were
inactivated. Therefore, the results shown in FIG. 13 indicate
that in order to obtain a greater inactivation of bacterial
spores, higher electric field strengths may be applied.

Accordingly, more than 95% of Bacillus subtilis bacterial
spores were inactivated by the continuous flow PEF treatment
system of the present invention with frequency ranges from 500
Hz to 4,000 Hz and a pulse duration time from 1 microseconds to
6 microseconds. Moreover, the optimum PEF treatment temperature
for Bacillus subtilis bacterial spores is 36.degree. C., and the
presence of a germination agent, such as L-alanine,
significantly enhances the PEF inactivation of the Bacillus
subtilis bacterial spores. Furthermore, frequencies beyond 4000
Hz are possible. For example, frequencies from 4000 to 20,000 Hz
may be employed depending on the system design parameters.

While the present invention has been illustrated by the
description of embodiments thereof, and while the embodiments
have been described in considerable detail, it is not the
intention of application to restrict or in any way limit the
scope of the appended claims to such detail. Additional
advantages and modifications will readily appear to those
skilled in the art. For example, the insulators, electrodes and
liquid flow chambers may employ a variety of cross-sectional and
sectional geometries, and the PEF treatment system operating
parameters, such as applied electric field strength, treatment
temperature and liquid product flow rates may be varied based on
the nature of the liquid product to be treated, the
micro-organism and/or bacterial to be inactivated. Therefore,
the invention, in its broader aspects, is not limited to the
specific details, the representative apparatus, and illustrative
examples shown and described. Accordingly, departures may be
made from such details without departing from the spirit or
scope of the applicant's general inventive concept.

---

**http://www.inventors-showcase.com/invention\_of\_the\_week**

**Invention of the Week --**

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---

  

**ULTRASONIC WINE AGING PATENTS**

**AGING OF ALCOHOLIC BEVERAGE****JPS5668385** PURPOSE:An alcoholic beverage is irradiated with ultrasonic
wave to accelerate the aging and maturing, thus producing
good-quality alcoholic beverage. CONSTITUTION:An alcoholic
baverage such as Japanese sake, whisky, wine, brandy or liquor is
irradiated with ultrasonic wave of 16-60KHz. The aging by the
irradiation with ultrasonic wave is though to be caused by
accelerating reactions of oxidation, polymerization, condensation,
etc., of alcohol, aldehydes, esters and olefins to form new
complicated substances that develop good flavor and body.  
**JPH0380070****RIPENING APPARATUS**PURPOSE:To provide a ripening apparatus effective in
accelerating the ripening of vinegar, etc., and composed of an
electromagnetic wave antenna, an ultrasonic vibrator and a far
infrared generation element placed on the inner wall of a tank or
a pipe and a driving apparatus to drive the above devices in a
continuous, intermittent or staggered mode. CONSTITUTION:Two or
more devices selected from an electromagnetic wave antenna 3, an
ultrasonic vibrator 4 and a far infrared generation element 5 are
placed on the inner wall of a tank 1 and the devices are driven in
continuous, intermittent or staggered mode with driving
apparatuses 8, 9, 10. A ripening object such as rice wine or
vinegar filled in the tank 1 is ripened in a short time by using
the ripening apparatus having the above construction.  
**ULTRASONIC APPARATUS FOR MANUFACTURING GINSENG WINE** **KR830000388** An ultrasonic appts. for manufg. Ginseng wine was described.A
transducer(6) was installed in treating chamber(5) to be high
density ultrasonic flux between A and B position. A mixing
chamber(2) was adjacently positioned to a evaporating chamber(12)
to circulate Ginseng wine by a pump(4). An exhaust pipe(13) and
cooling chamber(14) were positioned in the upper part of the
evaporating chamber, then sludges were removed in the storage
chamber(15). Thus, 30 kg dried Ginseng and 157l 95% spirits were
treated in 143 water for 6 hr to obtain Ginseng wine.  
**CN86200014** **WINE-MAKING DEVICE BY MEANS OF ION-IMPLANTATION**The utility model discloses a wine making device by means of
ion-implantation. Two hollow electrodes which are fixed by copper
sheet and the end are mutual isolation are inserted the relative
location of the sidewall of a treating groove. The other end of
the copper sheet is connected with high voltage power supply
through the switch of the sphere gap. When the utility model is
used, the oxygen enters the centre hole through the electrodes,
and the oxygen is ionized through high-pressure discharge when the
aging wine flow through the treating groove, so the aging process
of the wine is accelerated through the various effects, such as
oxidation, liquid electricity, strong light and ultrasonic wave.
The wine making device by means of ion-implantation has the
advantage of compact structure. The speed and the effect of the
aging wine is obviously better than the prior art.  
**JPH0795873****PRODUCTION OF WINE, BEER AND REFINED SAKE** PURPOSE:To provide a production method of wine, refined sake
(japapese rice wine), etc., capable of promoting fermentation and
production a fragrant product by irradiating weak ultrasonic wave
on the fermentation material at a low temperature in a
fermentation process. CONSTITUTION:Fermentation material is sealed
in a jar-fermenter 1 and the material is fermented while the
temperature of the material is controlled at <=15 deg.C by
means of a temperature controlling device 6. Vibration energy is
supplied to a piezoelectric transducer 3 from a vibration circuit
2 and weak ultrasonic wave is irradiated from the piezoelectric
transducer 3 into the inside of the jar-fermenter 1 to accelerate
the fermentation. In this process, owing to the irradiation of the
weak ultrasonic wave to the fermentation material at a low
temperature, the carbon dioxide is reduced to the saturated
concentration of the temperature and the fermentation is
stimulated. Fragrance components affecting favorable effect on the
product are increased, and accordingly, a fragrant fermentation
product can be produced.  
**JPS62269663** **RIPENING APPARATUS****BG51814** **Apparatus using ultrasound and replacing champagnisation of
sparkling wine****FR2687409** **HIGH EFFICIENCY ARTIFICIAL AGEING RIPENING DEVICE FOR WINE**Apparatus using ultrasound and replacing conventional
apparatuses for champagnisation of sparkling wines. Hanging ramps
on which the bottles are placed are loaded into the liquid in
which the ultrasonic transducer or transducers is or are placed.
An adjustable timer regulates the immersion time which is a few
seconds in length. The lamps are driven by chains, discs,
triangles or the like. The whole is connected to an electric
geared motor. Loading takes place on one side, unloading on the
other. Conventional champagnisation lasts several weeks. This
apparatus reduces the time to less than 48 hours.   
**CN2116697** **PRODUCTION OF WINE, BEER AND REFINED SAKE**The utility model relates to a high efficiency artificial
ageing ripening device for wine. The utility model comprises a
direct current electric source, an electrolyzing device for water
and an ageing ripening appliance, wherein, the voltage of the
direct current electric source is adjusted, the electrolyzing
device for water is used for an electrolyzing groove which is
provided with two groove chambers, and the ageing ripening
appliance cause oxygen and hydrogen generated by the electrolysis
of wine which is to be processed and water solution to play a
role. Furthermore, the ageing ripening appliance comprises a
housing and an air inlet pipe which are made of materials such as
stainless steel, ceramic or glass, etc., the inner part of the
ageing ripening appliance can also provided with an ultraviolet
lamp, an adsorption material with a plurality of holes, a
high-frequency coil, etc., and an ultrasonic device can also be
arranged on the ageing ripening appliance.  
  
****SET FOR CONTINUOUS PRODUCTION OF CLEARED JUICES AND WINES**JPH078236** **CN2183996** The utility model relates to a quickly wine brewing kettle,
comprising a wine containing container. The utility model is
characterized in that the utility model also has a set of
supersonic wave and laser emitting circuits; an ultrasonic
vibrator is arranged at the container wall; the emitting end of a
laser emitting tube is connected with an optical fiber bundle, the
tail end of the optical fiber bundle aims at the transparent dots
or the pinholes of the container wall. The utility model has the
advantages that the fermention and the catalysis of the wine can
be accelerated, the brewing time can be shortened, and the wine
can be made more palatable.  
**CN2213729** **Ultra-sonic treating device for liquid****US5604297** **Spirulina wine and preparing process thereof****CN1175632** **CN2280716** **IMPROVEMENT IN TASTE OF ALCOHOLIC BEVERAGE AND APPARATUS
THEREFOR**The utility model relates to an explosion-proof
multi-frequency-wave wine-ageing quickening device, which is
provided with an explosion-isolated regulation and control box. A
connection pipe is arranged on the explosion-isolated regulation
and control box, and the other end of the connection pipe is
provided with an explosion-isolated outer shell. An electronic
circuit board, an ultrasonic transducer and a super high frequency
coil are arranged in the explosion-isolated outer shell, and an
electrical conducting wire is arranged in the connection pipe and
connected with the explosion-isolated regulation and control box
and the electronic circuit board in the explosion-isolated outer
shell. The utility model can shorten the wine-ageing time, and has
the advantages of compact structure, low manufacture cost,
convenient use, etc.  
**JPH119257** **PLANT FOR STABILIZATION OF JUICES AND WINES****CN2393883** **WINE TREATMENT UNIT****JP2001186870** **METHOD FOR PRODUCING RED WINE**PROBLEM TO BE SOLVED: To provide a wine treatment unit
enabling wine flavor to be fully elicited in a short time at low
cost. SOLUTION: This wine treatment unit is designed to
continuously apply magnetism to a wine after vibrated, that is,
has such a scheme that a wine in the tank 11 set on an ultrasonic
vibrator 1 is subjected to ultrasonic vibration and then put to
magnetism continuously using a magnetism application unit
3.       
**GB2365417** **Portable wine device capable of removing harmful matter****CN2457163****Efficient wine-quality processing equipment****CN2522421** **A clarification and fusion method for low degree
distillation wine**An efficient wine-quality processing equipment uses
comprehensive processing means of high- and low-frequency
ultrasonic, ultraviolet light, constant magnetic magnetizer and so
on to process wine (liquor, beer, colored wine; more than ten
minutes are only needed to finish functions of eliminating
impurities, increasing fragrance and catalyzing the brewing
process; the brewing process is shortened to be between 1.5 years
and 2 years. The equipment has quite wide applicability; according
to needs of users, the equipment can be made into serial products
of various specifications, which is suitable for large and small
wineries; the equipment speeds up physical and chemical changes in
the brewing process of a novel wine and has functions of
''eliminating impurities'', ''increasing fragrance'', and
improving the taste of a novel wine; the equipment promotes the
early maturity of a novel wine and greatly improves the quality of
the novel wine.  
**JP2003289847** **TREATMENT OF A PRODUCT TO REDUCE PHENOLIC BITTERNESS** **WO03016459** **Ultrasonic vibration magnetizing appliance for liquid**A process is disclosed for treating a product, which will
usually be wine, which suffers from phenolic bitterness. The
method consists of treating the product with an ultrasonic
waveform, the waveform having a frequency of between 100 kHz and
1000 kHz and an intensity of between 1,2 W/cm squared and 100 W/cm
sqared. Preferably the energy dose is between 1000 and 100000 Watt
seconds per litre.  
**CN2597461** **HYPERSONIC DEVICE****RU2298030** **METHOD FOR PREPARING TRADITIONAL WINE USING PINE NEEDLES****NZ543156** **An improved active wine barrel****NZ543157** **Weak ultrasonic processor for liquid molecules****CN2747173** **Wine aging method and system****US2006289350** **Composite hastening aging equipment for wine****CN201167522** **ultrasonic salt atomization method for accelerating spirit
ageing****CN101223904** **Supersonic wave apparatus for aged wine****CN101294129** **Ultrasonic aging apparatus for wine****CN101422502** **CN101760412** **Wine comprehensive high-efficiency artificial strengthening
physical aging method and manufacturing method of aging machine
thereof****CN101760413****Cylindrical tube type acoustic liquid comprehensive
function coprocessor****CN201380070** **Device utilizing ultrasonic wave to promoting aging of
white wine****CN101525572** **An apparatus and method for the treatment of wine using
ultrasonic cavitations****CN201445912** **Fluid dynamic ultrasonic wine alcoholization device****CN101845379****Wine aging device****CN102051307** **Fermented red date wine and brewing method
thereof** **CN102051312****Pipeline type ultrasonic treatment device for promoting
ageing of wine** **CN201901668****CN102059070** **Hydraulic power/ ultrasonic coupling cavitation device****CN102181354** **Method and apparatus for rapidly ageing wine****CN202343099** **New technology for preparing ester spices by using fusel
oil enzyme method and natural ester spices****CN102559784****Nano instant ripe wine maker****CN102433254** **Novel technology for preparing natural
ethyl-3-methyl-thiopropionate spice and ester spice prepared by
novel technology** **CN102787058** **Method for brewing strawberry fruit wine by ultrasonic
waves****CN103013735****Fermentation technology of honey wine****CN102994302****Preparation technology of cordyceps militaris grape wine****CN103053471** **Ultrasonic treatment device and method for reducing ethyl
carbamate in brewed wine****CN103103102****Brewing method for healthcare mulberry fruit wine**  
The invention discloses an ultrasonic treatment device for
reducing ethyl carbamate (EC) in brewed wine. The ultrasonic
treatment device comprises a rotational ultrasonic generator (11),
wherein the lower part of the rotational ultrasonic generator has
a structure which is in the shape of a Chinese character 'feng'; a
motor (6) and a speed reducing box (7) are arranged above the
rotational ultrasonic generator; the line incoming side of the
motor is connected with a time controller (8); the whole device is
vertically arranged in the center of a tank body (1); the tank
body has a jacket-type two-layered wall structure; an interlayer
between the two layers of walls is a water filling layer (10); a
water outlet (12) with a valve is formed in the lower part of the
outer wall; a water inlet (2) with a valve, a heating pipe jack
(3) and a tank cover (5) are arranged on the upper part of the
outer wall; a feeding port (9) with a valve is formed in the upper
part of the tank cover; and a discharging port (13) with a valve
is arranged on the lower part of the tank body. The reduction of
EC is carried out in three steps: 1, preparation of pretreatment;
2, introduction of wine to be treated and adding of relevant
materials; and 3, treatment of reducing the EC. By using the
device and the method, 30-50 percent of EC in the wine can be
reduced, and the health of drinkers is facilitated; and the device
is low in energy consumption, low in cost and small in pollution.  
  


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